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Diagram illustrating estrus synchronization procedures. Short-term progesterone estrus synchronization protocols using PMSG and PMSG-loaded chitosan nanoparticles were implemented. In Group 1, ewes were synchronized with 1 mL of Lutone (25 mg of progesterone acetate in oil) daily for 7 consecutive days. On the 6th day, they received 600 IU of PMSG (Folligon®), followed by 1 mL of Estrumate (250 μg of PGF 2α ) on the 7th day. Estrus detection was conducted with <t>an</t> <t>intramuscular</t> injection of 1 mL of Receptal (4 μg of <t>GnRH)</t> at breeding. In Group 2, the treatment regimen was the same as Group 1, except that on the 6th day, ewes received 300 IU of PMSG-loaded chitosan nanoparticles. Group 3 followed a treatment protocol similar to Groups 1 and 2, except for administering 150 IU of PMSG-loaded chitosan nanoparticles on the 6th day.
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Diagram illustrating estrus synchronization procedures. Short-term progesterone estrus synchronization protocols using PMSG and PMSG-loaded chitosan nanoparticles were implemented. In Group 1, ewes were synchronized with 1 mL of Lutone (25 mg of progesterone acetate in oil) daily for 7 consecutive days. On the 6th day, they received 600 IU of PMSG (Folligon®), followed by 1 mL of Estrumate (250 μg of PGF 2α ) on the 7th day. Estrus detection was conducted with <t>an</t> <t>intramuscular</t> injection of 1 mL of Receptal (4 μg of <t>GnRH)</t> at breeding. In Group 2, the treatment regimen was the same as Group 1, except that on the 6th day, ewes received 300 IU of PMSG-loaded chitosan nanoparticles. Group 3 followed a treatment protocol similar to Groups 1 and 2, except for administering 150 IU of PMSG-loaded chitosan nanoparticles on the 6th day.
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Diagram illustrating estrus synchronization procedures. Short-term progesterone estrus synchronization protocols using PMSG and PMSG-loaded chitosan nanoparticles were implemented. In Group 1, ewes were synchronized with 1 mL of Lutone (25 mg of progesterone acetate in oil) daily for 7 consecutive days. On the 6th day, they received 600 IU of PMSG (Folligon®), followed by 1 mL of Estrumate (250 μg of PGF 2α ) on the 7th day. Estrus detection was conducted with <t>an</t> <t>intramuscular</t> injection of 1 mL of Receptal (4 μg of <t>GnRH)</t> at breeding. In Group 2, the treatment regimen was the same as Group 1, except that on the 6th day, ewes received 300 IU of PMSG-loaded chitosan nanoparticles. Group 3 followed a treatment protocol similar to Groups 1 and 2, except for administering 150 IU of PMSG-loaded chitosan nanoparticles on the 6th day.
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Diagram illustrating estrus synchronization procedures. Short-term progesterone estrus synchronization protocols using PMSG and PMSG-loaded chitosan nanoparticles were implemented. In Group 1, ewes were synchronized with 1 mL of Lutone (25 mg of progesterone acetate in oil) daily for 7 consecutive days. On the 6th day, they received 600 IU of PMSG (Folligon®), followed by 1 mL of Estrumate (250 μg of PGF 2α ) on the 7th day. Estrus detection was conducted with <t>an</t> <t>intramuscular</t> injection of 1 mL of Receptal (4 μg of <t>GnRH)</t> at breeding. In Group 2, the treatment regimen was the same as Group 1, except that on the 6th day, ewes received 300 IU of PMSG-loaded chitosan nanoparticles. Group 3 followed a treatment protocol similar to Groups 1 and 2, except for administering 150 IU of PMSG-loaded chitosan nanoparticles on the 6th day.
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Diagram illustrating estrus synchronization procedures. Short-term progesterone estrus synchronization protocols using PMSG and PMSG-loaded chitosan nanoparticles were implemented. In Group 1, ewes were synchronized with 1 mL of Lutone (25 mg of progesterone acetate in oil) daily for 7 consecutive days. On the 6th day, they received 600 IU of PMSG (Folligon®), followed by 1 mL of Estrumate (250 μg of PGF 2α ) on the 7th day. Estrus detection was conducted with an intramuscular injection of 1 mL of Receptal (4 μg of GnRH) at breeding. In Group 2, the treatment regimen was the same as Group 1, except that on the 6th day, ewes received 300 IU of PMSG-loaded chitosan nanoparticles. Group 3 followed a treatment protocol similar to Groups 1 and 2, except for administering 150 IU of PMSG-loaded chitosan nanoparticles on the 6th day.

Journal: Frontiers in Veterinary Science

Article Title: Improving the breeding capabilities of short-term estrus synchronized Ossimi sheep using pregnant mare serum gonadotropin loaded chitosan-nanoparticles

doi: 10.3389/fvets.2025.1607674

Figure Lengend Snippet: Diagram illustrating estrus synchronization procedures. Short-term progesterone estrus synchronization protocols using PMSG and PMSG-loaded chitosan nanoparticles were implemented. In Group 1, ewes were synchronized with 1 mL of Lutone (25 mg of progesterone acetate in oil) daily for 7 consecutive days. On the 6th day, they received 600 IU of PMSG (Folligon®), followed by 1 mL of Estrumate (250 μg of PGF 2α ) on the 7th day. Estrus detection was conducted with an intramuscular injection of 1 mL of Receptal (4 μg of GnRH) at breeding. In Group 2, the treatment regimen was the same as Group 1, except that on the 6th day, ewes received 300 IU of PMSG-loaded chitosan nanoparticles. Group 3 followed a treatment protocol similar to Groups 1 and 2, except for administering 150 IU of PMSG-loaded chitosan nanoparticles on the 6th day.

Article Snippet: Estrus detection was conducted from the last day to the 11th day, with an intramuscular injection of 4 μg of GnRH [1 mL Receptal (GnRH analog, buserelin acetate, Intervet International, Netherlands)] at breeding, as illustrated in .

Techniques: Injection